Natural substances have been of interest for several centuries, and many toxins of animal, plant, bacterial, or fungal origins have been identified and characterized. There is a long way to go, from finding venomous species in their natural habitat, through collecting their venom under strict safety and species preservation conditions, to the extraction of natural toxins in the laboratory. State-of-the-art technologies are making it possible to explore, as close as possible to their site of action, the functioning of these tools on a molecular level and to design some of them that will contribute to the development and progress of diagnostic medicine or therapeutics. In this Special Issue of the journal Toxins, titled “Toxins: From the Wild to the Lab”, research and review articles concerning the latest discoveries in the field of venoms and toxinology will be presented to cover broad areas ranging from the composition and evolution of venoms to the mechanism of action, structural conservation versus variability, and therapeutic applicability of toxins. This Special Issue is open, albeit not restricted, to the communications that were presented during the 29th Meeting of Toxinology (RT29) organized by the French Society of Toxinology (SFET) on the 30th of November and 1st of December 2023 at the Institut Pasteur of Paris.